Thin-layer agarose electrophoresis of lactate dehydrogenase isoenzymes in serum: a note on the method of reporting and on the lactate dehydrogenase isoenzyme-1/isoenzyme-2 ratio in acute myocardial infarction.
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Thin-layer agarose electrophoresis of lactate dehydrogenase isoenzymes in serum: a note on the method of reporting and on the lactate dehydrogenase isoenzyme-1/isoenzyme-2 ratio in acute myocardial infarction.
We assessed the clinical efficacy of a thin-layer agarose electrophoresis assay for lactate dehydrogenase isoenzyme estimation in the diagnosis of acute myocardial infarction. From a population of 228 patients admitted to the Coronary Care Unit with suspected acute myocardial infarction, all 101 positive cases (confirmed by clinical presentation and electrocardiographic changes) were correctly ...
متن کاملSerum lactate dehydrogenase isoenzymes in the diagnosis of myocardial infarction.
The ratio of lactate dehydrogenase (LD-1:LD-2) in serum was studied in 101 patients admitted to a coronary care unit. The clinical reliability of the test could not be predicted from the normality or abnormality of the total serum lactate dehydrogenase (LD) activity. However, for samples with total LD activity below the middle of the normal range, no information was provided by the test. In con...
متن کاملInfluence of hemolysis on the serum lactate dehydrogenase-1/lactate dehydrogenase-2 ratio as determined by an accurate thin-layer agarose electrophoresis procedure.
The accuracy of our lactate dehydrogenase (EC 1.1.1.27) isoenzyme procedure, based on electrophoresis in thin-layer agarose, has previously been assessed (Clin. Chem. 22: 1995, 1976). We now show that the method is similarly accurate and precise for the specific measurements of lactate dehydrogenase isoenzyme-1 and -2. We have also determined the lactate dehydrogenase isoenzyme pattern in perip...
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1. Papadopoulos, N. M., and Hufnagel, C. A., Evaluation of lactate dehydrogenase isoenzyme patterns in serum of patients undergoing cardiac surgery. J. Thorac. Cardiovasc. Surg. 76, 173-177 (1978). 2. McKenzie, D., Clarke, P. I., and Henderson, A. R., How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique? Clin. Chem. 22, 1995-1998 (1976). 3...
متن کاملGlucose estimated with a Beckman Glucose Analyzer 2 in spots of capillary blood on filter paper.
1. Papadopoulos, N. M., and Hufnagel, C. A., Evaluation of lactate dehydrogenase isoenzyme patterns in serum of patients undergoing cardiac surgery. J. Thorac. Cardiovasc. Surg. 76, 173-177 (1978). 2. McKenzie, D., Clarke, P. I., and Henderson, A. R., How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique? Clin. Chem. 22, 1995-1998 (1976). 3...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1979
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/25.2.209